Thermo Fisher Scientific Connexin 43 Recombinant Rabbit Monoclonal Antibody (105)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:500

Product Specifications

Target Information

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as hexamers and are transported to the plasma membrane to create hemichannels that can associate with hemichannels on nearby cells to form cell-to-cell channels. Clusters of these channels assemble to make gap junctions, which are important in development and regulation of cell growth.

Phosphorylation of Cx43 regulates assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, decreasing cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells.

Based on sequence similarities, gap junction proteins are divided into alpha and beta categories. Connexin 43 is the major protein of gap junctions in the heart and plays a crucial role in synchronized cardiac contraction and embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, oculodentodigital dysplasia, and heart malformations. Alternatively spliced transcript variants of GFAP have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.