Thermo Fisher Scientific Epi5 Episomal iPSC Reprogramming Kit

Invitrogen™ Epi5™ Episomal iPSC Reprogramming Kit

The Epi5™ Episomal iPSC Reprogramming Kit provides an easy-to-use, highly efficient set of five episomal vectors designed by Dr. Okita in the laboratory of Professor Yamanaka at the Center for iPS Cell Research and Application (CiRA), Kyoto University.
This system produces transgene-free, virus-free human induced pluripotent stem cells (iPSCs) with efficiencies ranging from 0.04% to 0.3%, depending on the cell type being reprogrammed.

주요 특징

• 간편하고 높은 효율성: 소분자 첨가제 없이 리프로그래밍 가능하며, p53 억제를 통한 iPSC 생성 효율 향상
• 트랜스진 및 바이러스 프리 시스템: 기본 연구부터 전임상 연구까지 사용 가능
• 유연한 미디어 시스템: feeder-free 또는 feeder 기반 시스템 모두에 적용 가능

Create Transgene and Virus-free iPSCs

Episomal vectors are a well-established system for producing transgene-free, virus-free iPSCs, providing a reliable source of iPSCs for all stages of pluripotent stem cell research.
Other reprogramming methods, such as lentivirus, may integrate transgenes into the host genome, potentially causing genomic disruption or unpredictable results.

As oriP/EBNA1 vectors, these episomal vectors contain five reprogramming factors (Oct4, Sox2, Lin28, Klf4, and L-Myc) and replicate extra-chromosomally once per cell cycle.
At this replication rate, episomes are lost at approximately 5% per cell generation.
This system enhances iPSC generation through p53 suppression, and the inclusion of L-Myc has been shown to be more potent and specific than c-Myc in human iPSC generation (1).

Easy-to-Use System

This two-vial kit provides an optimal system for episomal vector reprogramming and eliminates the need for small molecules in the reprogramming medium.
The episomal vectors are introduced into cells by electroporation.
Use of the Neon™ Transfection System is recommended for electroporation, offering flexibility in volume and cell number required for reprogramming.

Flexibility in Media Systems

The Epi5™ Episomal iPSC Reprogramming Kit supports multiple cell types and media systems.
Protocols are available for fibroblasts and CD34+ blood cells in both feeder and feeder-free conditions.

참고 문헌

1. Okita K, Matsumara Y, Sato Y, et al. A more efficient method to generate integration-free human iPS cells. Nature Methods, 8, 409–412 (2011).

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