
Thermo Fisher Scientific Chicken anti-Goat IgG (H+L) Secondary Antibody, HRP
닭에서 유래한 항염소 IgG(H+L) HRP 결합 2차항체로, ELISA, Western blot, IHC 등 다양한 응용에 적합. 친화 크로마토그래피로 정제되었으며 높은 특이성과 감도를 제공. 동결건조 형태로 제공되어 안정적 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2534637 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats
- AP
- Biotin
- FITC
- TRITC
- Custom conjugation available upon request
Product Specific Information
Each antibody lot is tested for sensitivity by ELISA and specificity by immunoelectrophoresis (IEP).
Rehydrate with 1.1 mL of deionized water and allow to stand for 30 minutes at room temperature to dissolve (product overfilled for complete recovery). Centrifuge to remove particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8°C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin goat serum components.
Target Information
Anti-Goat secondary antibodies are affinity-purified and exhibit well-characterized specificity for goat immunoglobulins.
They are useful for detection, sorting, or purification of goat IgG and compatible with various detection systems such as HRP, AP, and fluorescence.
These antibodies enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically generated by immunizing host animals with pooled goat immunoglobulins, they may be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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