Invitrogen™

Epi5™ Episomal iPSC Reprogramming Kit

The Epi5™ Episomal iPSC Reprogramming Kit provides an easy-to-use, highly efficient set of 5 episomal vectors designed by Dr. Okita자세히 알아보기

The Epi5™ Episomal iPSC Reprogramming Kit provides an easy-to-use, highly efficient set of 5 episomal vectors designed by Dr. Okita in the laboratory of Professor Yamanaka at the Center for iPS Cell Research and Application (CiRA), Kyoto University. This system produces transgene-free, virus-free human induced pluripotent stem cells (iPSCs) with efficiencies in the range of 0.04% to 0.3%, depending upon the cell type being reprogrammed.

Features of the Epi5™ Episomal iPSC Reprogramming Kit include:

• Easy to use and highly efficient—no need for small molecules during reprogramming and the added p53 suppression provides enhanced iPSC generation (1)
• Usable for all stages of your iPSC research—transgene-free and viral-free reprogramming allows use from basic to pre-clinical research
• Flexibility in media systems—can be used in either feeder-free or feeder-based media systems

Create Transgene and Virus-free iPSCs
Episomal vectors are a well-described system for producing transgene-free, virus-free iPSCs, providing a source of iPSCs for all stages of your pluripotent stem cell research. Other reprogramming methods, such as lentivirus, contain transgenes that can integrate into the host genome, potentially disrupting the genome or causing unpredictable results.

As oriP/EBNA1 vectors, these episomal vectors contain 5 reprogramming factors (Oct4, Sox2, Lin28, Klf4, and L-Myc) and replicate extra-chromosomally only once per cell cycle. At this replication rate, the episomes are lost at a rate of approximately 5% per cell generation. This system shows enhanced iPSC generation through p53 suppression, and the inclusion of L-Myc has been shown to be more potent and specific then c-Myc during human iPSC generation (1).

Easy-to-Use System
This 2-vial kit provides the optimal system for episomal vector reprogramming and eliminates the need for use of small molecules in the reprogramming medium. The episomal vectors are introduced into the cell by electroporation. We recommend use of the Neon™Transfection System for electroporation, allowing flexibility in the volume and thus number of cells needed for reprogramming.

Flexibility in Selection of Media Systems
The Epi5™ Episomal iPSC Reprogramming Kit can be used with multiple cell types and media systems. Protocols for fibroblasts and CD34+ blood cells are available in either feeder or feeder-free based reprogramming.

1. Okita K, Matsumara Y, Sato Y, et al. A more efficient method to generate integration free human iPS cells. Nature Methods 8, 409-412, 2011.

사양

클로닝 방법None

배달 유형Transfection

용도(애플리케이션)Stem Cell Engineering & Reprogramming

용도(장비)Electroporator, Neon Transfection System

주요 기능el

제품라인Epi5™

제품 유형Episomal iPSC Reprogramming Kit

프로모터CAG

단백질 태그None

수량1 kit

복제 개시점pUC Origin

리포터 유전자None

선택 제제(진핵)None

벡터EBNA OriP

Unit SizeEach