Thermo Fisher Scientific Phospho-Histone H3 (Ser28) Monoclonal Antibody (HTA28), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific Phospho-Histone H3 (Ser28) Monoclonal Antibody (HTA28), Alexa Fluor 488, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: N/A

Product Specifications

Product Specific Information

The HTA28 monoclonal antibody recognizes phosphorylated serine 28 of histone H3 in human, mouse, rat, bovine, and hamster. Histone H3 (15 kDa) is a chromatin component forming nucleosome structure. Phosphorylation at Ser28 is mediated by MSK1 following MAP kinase activation in response to tumor promoters (UV, EGF) and oncoproteins (c-Myc, c-Jun, c-Fos). This modification is associated with chromosome condensation during mitosis, cell transformation, and RNA polymerase III transcription regulation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated for intracellular staining of nocodazole-treated HeLa cells (≤ 0.5 µg/test). A test is defined as the antibody amount for staining a 100 µL cell sample (10⁵–10⁸ cells/test). Optimize titration for best results.

Recommended Protocols:

• Protocol A: Two-step (intracellular/cytoplasmic proteins)
• Protocol C: Two-step (Fixation/Methanol)
• Protocol B (one-step, nuclear proteins) cannot be used.
  Detailed instructions are available in BestProtocols® – Staining Intracellular Antigens for Flow Cytometry.

Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser

Target Information

Histone H3 is a DNA-binding protein forming nucleosomes with other core histones. It regulates transcription, DNA repair, replication, and chromosomal stability. The N-terminal tail undergoes post-translational modifications such as methylation, acetylation, and phosphorylation, influencing chromatin structure and transcription factor binding.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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