Thermo Fisher Scientific Phospho-Histone H3 (Ser28) Monoclonal Antibody (HTA28), Alexa Fluor 488, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HTA28

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• eFluor 660
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2784758

Product Specific Information

Description: The HTA28 monoclonal antibody recognizes phosphorylated serine 28 of human, mouse, rat, bovine, and hamster histone H3. This 15 kDa protein is a component of eukaryotic chromatin that is involved in forming the nucleosome structure. Histone H3 can be phosphorylated at serine 10 and serine 28. Studies have demonstrated that phosphorylation at serine 28 is mediated by MSK1 following activation by the MAP kinase signaling pathway in response to tumor promoters (e.g., UV and EGF) and oncoproteins (e.g., c-Myc, c-Jun, and c-Fos). Histone H3 serine 28 phosphorylation has been linked to chromosome condensation during mitosis, cell transformation, and regulation of RNA polymerase III transcription machinery.

Applications Reported: This HTA28 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HTA28 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of nocodazole-treated HeLa cells This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Protocols: We recommend using Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins or Protocol C: Two-step protocol: Fixation/Methanol. Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Staining intracellular Antigens for Flow Cytometry Protocol located in the BestProtocols® Section under the Resources tab online.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.