Thermo Fisher Scientific Pan Cytokeratin Monoclonal Antibody (AE1/AE3), Alexa Fluor 488, eBioscience
Alexa Fluor 488 결합된 AE1/AE3 단클론 항체로 다양한 세포각질 단백질을 인식합니다. 면역형광, 면역조직화학 등 여러 응용에 적합하며, 인간 및 다양한 종에서 반응성을 보입니다. 0.5 mg/mL 농도의 액상 형태로 제공되며, 4°C 암소 보관을 권장합니다.
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Thermo Fisher Scientific Pan Cytokeratin Monoclonal Antibody (AE1/AE3), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | 1 publication |
| Immunohistochemistry (IHC) | - | 22 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | 4 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | 2 publications |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | 20 publications |
| Flow Cytometry (Flow) | - | 8 publications |
| in situ PLA (PLA) | - | 1 publication |
| Inhibition Assays (IA) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Non-human primate, Rabbit, Rhesus monkey |
| Published Species | Human, Mouse, Pig, Rabbit |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AE1/AE3 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation/Emission Max | 499/520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1834351 |
Product Specific Information
The monoclonal antibodies AE1 and AE3 recognize many acidic and basic cytokeratin family members. Cytokeratins are intermediate filament proteins forming part of the cytoskeleton, expressed in epithelial cells and developmentally regulated. Tumors often express these proteins, aiding in the identification of neoplasm origin.
- AE3 antibody recognizes 65–67 triplet, 64, 59, 58, 56, 54, and 52 kDa proteins (cytokeratin 1–8).
- AE1 antibody recognizes 56.5, 54, 50, 48, and 40 kDa proteins (CK10, 14, 15, 16, 19).
Applicable to tissue samples from mouse, human, rat, primates, dog, cat, rabbit, and chicken.
Applications Reported: Immunohistochemical staining, immunocytochemistry, frozen and paraffin-embedded tissue staining.
Applications Tested: Immunofluorescent staining of formaldehyde-fixed or methanol-fixed MCF7 cells at ≤1 µg/mL.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Cytokeratin pan belongs to a subfamily of intermediate filament proteins with biochemical diversity, represented by at least 20 human epithelial polypeptides (40–68 kDa, pI 4.9–7.8). Expression patterns vary by epithelial type and differentiation stage, serving as markers for distinguishing epithelial malignancies.
Cytokeratin antibodies assist in tumor differential diagnosis via immunohistochemistry and are also used in cytopathology and flow cytometry. Their composition and expression vary with cell type, growth environment, and disease state, making them valuable in cancer research and tumor diagnostics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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