Norgen DNA Clean-Up and Concentration Micro-Elute Kit

Norgen DNA Clean-Up and Concentration Micro-Elute Kit

SKU: 67200
For the purification, cleanup and concentration of DNA

Features and Benefits

• Rapid concentration of small amounts of DNA into flexible final elution volumes of 8–15 μL
• Ideal for concentrating DNA from PCR and other enzymatic or labeling reactions, and cleanup of plasmids or DNA isolated by other methods
• Isolated DNA is suitable for downstream applications including PCR, sequencing, ligation, RNA transcription, radiolabeling, arrays, and more

Product Description

Norgen’s DNA Clean-Up and Concentration Micro-Elute Kit provides a rapid method for the purification, cleanup, and concentration of up to 40 μg of DNA from PCR reactions, endonuclease digestions, DNA modification or labeling reactions, and more.
Plasmids (<10,000 bp) and genomic DNA can also be concentrated.
The minimum recommended elution volume is 8 μL, enabling concentration of small amounts of DNA of all sizes.

The DNA is purified from proteins, nucleases, free nucleotides, and other contaminants. The purified DNA is salt-free and ready for use in PCR, sequencing, ligation, endonuclease digestion, radiolabeling, and array applications.
Preparation time per sample: ~20 minutes. Each kit supports 50 preparations.

Supporting Data

Figure 1: Efficient Concentration of DNA from Various Sources
Approximately 25 µg of plasmid DNA (A), HEK 293 cell line DNA (B), or PCR product (C) was concentrated and eluted into 15 µL.
1 µL of unconcentrated (U) and concentrated DNA (C) was run on a 1.3% agarose gel at 170 V for 25 min.
The kit shows significantly higher final DNA concentration.
M = Norgen HighRanger DNA Ladder (Cat#11900)

Norgen’s Purification Technology

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix.
DNA is purified without phenol or chloroform. The process includes:

1. Mixing DNA samples with Buffer RL
2. Adding ethanol and loading onto an activated spin column
3. Binding DNA to the resin
4. Washing three times with Wash Solution A
5. Eluting purified DNA into 8–15 μL using Elution Buffer F

Kit Specifications

Advantages

• Concentrates small DNA amounts into 8–15 μL
• Ideal for PCR and enzymatic labeling cleanup
• No organic solvents required
• Compatible with DNA from other isolation methods
• Fast, easy spin-column format
• Suitable for DNA from 50 bp to 10,000 bp

Kit Components

Storage Conditions and Stability

All solutions should be stored tightly sealed at room temperature.
Stable for at least 2 years in unopened containers.

Documentation

DNA Clean-Up and Concentration Micro-Elute Kit - Protocol (50 preps)

FAQs

Why do I have poor DNA recovery?

• Column clogged: Do not exceed recommended input amounts.
• Alternative elution used: Use supplied Elution Buffer F.
• Ethanol missing in lysate or wash buffer: Ensure ethanol addition before binding and washing.

Why is the column clogged?

• Too much DNA input: ≤40 μg per column.
• Low centrifuge temperature: Keep centrifuge at room temperature (>15°C).

Why is the eluted DNA degraded?

• DNase contamination: Use sterile technique.
• DNA size >10,000 bp: May shear during purification.
• Improper storage: Store at 4°C short-term, –20°C long-term.

Why is the purified DNA not performing well?

• Insufficient washing: Perform three washes with Wash Solution A.
• Ethanol carryover: Ensure dry spin step.
• Improper pH: Elution buffer pH should be 7–8.
• Inadequate mixing: Thoroughly mix sample and binding buffer.

Citations

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