Thermo Fisher Scientific ATP7B Monoclonal Antibody (L62/29), FITC
ATP7B 단백질을 인식하는 FITC 결합 단일클론 항체로, Western blot, IHC, ICC/IF, IP 등 다양한 응용에 사용 가능. 사람, 생쥐, 랫트 반응성. 단백질 G 정제, 보존제 없음, 4°C 암소 보관. Wilson 병 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | L62/29 |
| Immunogen | Synthetic peptide amino acids 3–21 (cytoplasmic N-terminus) of human Copper-transporting ATPase2 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS (pH 7.4), with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931977 |
Additional Formats
- Unconjugated (MA5-27719)
- APC (MA5-45522)
- PE (MA5-45525)
- PerCP (MA5-45524)
- Request custom conjugation
Product Specific Information
- 1 µg/mL of MA5-45523 was sufficient for detection of Copper-transporting ATPase2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.
- Detects approximately 160 kDa in rat brain membrane preparations.
- This antibody was formerly sold as clone S62-29.
Target Information
This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least two putative copper-binding sites.
This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile.
Alternate transcriptional splice variants encoding different isoforms with distinct cellular localizations have been characterized.
Mutations in this gene have been associated with Wilson disease (WD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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