Thermo Fisher Scientific ATP7A Monoclonal Antibody (L60/4), FITC
ATP7A 단백질 검출용 FITC 결합 단일클론 항체로, 인간·마우스·랫트 시료에 반응. WB, IHC, ICC 등 다양한 분석에 사용 가능. 498/517 nm 형광 특성. Protein G 정제, 보존제 무첨가, 4°C 암소 보관.
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Thermo Fisher Scientific ATP7A Monoclonal Antibody (L60/4), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | L60/4 |
| Immunogen | Synthetic peptide amino acids 42–61 (cytoplasmic C-terminus) of human Copper-transporting ATPase1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931973 |
Additional Formats
Product Specific Information
- 1 µg/mL of MA5-45519 was sufficient for detection of Copper-transporting ATPase1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.
- Detects approximately 180 kDa in rat brain membrane preparations.
- This antibody was formerly sold as clone S60-4.
Target Information
ATP7A (Copper-transporting ATPase 1) is a transmembrane copper-translocating P-type ATPase essential for systemic copper absorption in the gut and reabsorption in the kidney. It is expressed in many tumor types and its upregulation confers resistance to cisplatin and carboplatin. Mutations in ATP7A cause Menkes disease, a fatal disorder in early childhood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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