Thermo Fisher Scientific EAAC1 Recombinant Rabbit Monoclonal Antibody (JU39-69)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JU39-69

Immunogen

Recombinant protein within Human EAAT3 aa 80-209 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EAAC1,uniProtId:P43005-1,ncbiNodeId:9606,antigenRange:80-209,antigenLength:524,antigenImageFileName:MA5-34614_EAAC1_P43005-1_Rabbit.svg,antigenImageFileNamePDP:MA5-34614_EAAC1_P43005-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Shipping conditions

Wet ice

RRID

AB_2848522

Product Specific Information

Positive Control: Mouse liver tissue lysate, HUVEC, LOVO, SH-SY5Y, rat brain tissue, human tonsil tissue, human colon cancer tissue, human kidney tissue.Cell membrane.

Target Information

L-glutamate is the major excitatory neurotransmitter in mammalian central nervous systems. To maintain glutamate levels below excitoxic levels, excess glutamate at excitatory synaptic clefts is removed by ion-coupled glutamate transporters. Four glutamate transporters have been identified: the sodium-dependent GLAST-1, GLT-1, EAAC-1 and the chloride-dependent EAAT-4. EAAC-1 is expressed in the CNS and also found in kidney and small intestine. Structural features of glutamate transporters are believed to include eight membrane-spanning alpha-helices and a loop-pore structure which is unique among secondary transporters but may resemble loop-pores found in ion channels. A second distinctive structural feature is the presence of a highly amphipathic membrane-spanning helix that provides a hydrophilic path through the membrane. Mice with homozygous deletions of the EAAC-1 transporter gene develop dicarboxylic aminoaciduria and display reduced spontaneous locomotor activity although no neurodegeneration was observed over a period of 12 months.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.