Thermo Fisher Scientific KCNQ1 Monoclonal Antibody (N37A/10), PerCP
KCNQ1 단백질을 인식하는 Mouse monoclonal antibody (Clone N37A/10)로 PerCP 형광 표지. WB, IHC, ICC, IP 등 다양한 응용에 사용 가능. Human, Mouse, Rat, Hamster 반응성. Protein G 정제, 1 mg/mL 농도, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| Immunoprecipitation (IP) | Assay-dependent | |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N37A/10 |
| Immunogen | Fusion protein amino acids 2–101 of human KCNQ1 |
| Conjugate | PerCP |
| Excitation/Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931863 |
Available Formats
Product Specific Information
1 µg/mL of MA5-45409 was sufficient for detection of KCNQ1 in 10 µg of COS-1 cell lysate transiently expressing KCNQ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 75 kDa.
Target Information
Voltage-gated K⁺ channels in the plasma membrane control the repolarization and frequency of action potentials in neurons, muscles, and other excitable cells.
The KCNQ1 alpha subunit and KCNE1 beta subunit form a specific K⁺ channel predominantly expressed in the heart and cochlea, regulating the slow, depolarization-activated potassium current.
Mutations in KCNQ1 and KCNE1 cause cardiac disease due to impaired electrical signaling, while KCNQ4 mutations are linked to deafness.
KCNQ proteins have six transmembrane domains and function as tetramers; KCNQ4 forms heteromeric channels with KCNQ3 and is expressed in tissues including the cochlea.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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