Thermo Fisher Scientific CD66a (CEACAM1) Monoclonal Antibody (CC1), PE-Cyanine7, eBioscience
CD66a(CEACAM1)을 인식하는 마우스 단클론 항체로 PE-Cyanine7 형광으로 결합됨. Flow cytometry에 최적화되어 있으며 0.125 µg/test로 사용 가능. B세포 및 다양한 면역세포 표면 단백질 분석에 적합. 4°C 어둠에서 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | – | View 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CC1 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762703 |
Available Formats:
Product Specific Information
The monoclonal antibody CC1 recognizes CD66a (CEACAM1), also known as carcinoembryonic antigen-related cell adhesion molecule 1, biliary glycoprotein, and BPG.
Expression of CD66a is found on brush borders, epithelial, and endothelial cells. In hematopoietic cells, expression is abundant on B cells, NK cells, monocytes, dendritic cells, and granulocytes.
Resting mouse T lymphocytes do not express CD66a, as confirmed by lack of staining with CC1 antibody. In humans, CD66a expression levels have been used to identify malignancies and play a key role in regulating BCR activation in B lymphocytes.
An alternate allele, CEACAM1b, is expressed in SJL mice; therefore, CC1 does not stain SJL tissue.
The CC1 clone has been shown to block viral infection and enhance B cell proliferation when combined with IgM crosslinking.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes (≤0.125 µg/test).
A test is defined as the amount of antibody that will stain a cell sample in 100 µL final volume. Recommended to titrate for optimal performance.
Light Sensitivity: PE-Cyanine7 is sensitive to photo-induced oxidation. Protect vial and samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark.
Excitation: 488–561 nm
Emission: 775 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Target Information
CEACAM1 is a member of the carcinoembryonic antigen (CEA) gene family within the immunoglobulin superfamily.
It functions as a cell-cell adhesion molecule expressed on leukocytes, epithelia, and endothelia.
CEACAM1 mediates homophilic and heterophilic binding and is involved in tissue organization, angiogenesis, apoptosis, tumor suppression, metastasis, and immune modulation.
Multiple transcript variants and isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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