Thermo Fisher Scientific Apolipoprotein B Monoclonal Antibody (1606)

Applications

• Radioimmune Assays (RIA): Assay-dependent

Product Specifications

Product Specific Information

MA1-27262 detects Apolipoprotein B from human samples.

Target Information

Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL) with a molecular mass of approximately 260–500 kDa. Apolipoprotein B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation.
Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and mediates the uptake and clearance of cholesterol from circulation.
In atherosclerotic lesions, oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis, though the precise changes in oxidized LDL are not well described.
Apolipoprotein B is mostly synthesized in the liver and is a major apolipoprotein of very low density, intermediate density, and low density lipoproteins (LDL), as well as a major component of lipoprotein (a).
Apolipoprotein B is a ligand for the LDL receptor, and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800 mg/L.
Apolipoprotein B occurs in plasma as two main isoforms, apoB-48 and apoB-100. The intestinal and hepatic forms are encoded by a single gene from a single long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA→UAA), creating a stop codon and early translation termination.
Mutations in the Apolipoprotein B gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective Apolipoprotein B, affecting plasma cholesterol and Apolipoprotein B levels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.