Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (VU-1D9), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

-

Immunohistochemistry (IHC)

Assay-Dependent

-

Flow Cytometry (Flow)

Assay-Dependent

-

Immunoprecipitation (IP)

Assay-Dependent

-

Functional Assay (Functional)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

VU-1D9

Immunogen

Rec. Human EpCAM (CD326)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10597285

Product Specific Information

Description: BMS171 (VU-1D9) specifically reacts with human Ep-CAM, a 40kD epithelial protein expressed on baso-lateral cell surface in many epithelial tissues (but absent from mesothelial tissues).

BMS171 differentiates between mesothelioma and carcinoma, a common diagnostic problem.

EpCAM-1 is a homophilic and Ca2+ independent adhesion molecule that is expressed on the baso-lateral cell surface of many epithelial, but not mesothelial tissues. It is expressed de novo in squamous cell carcinoma but is absent in the majority of healthy squamous epithelia. EpCAM expression correlates with cell proliferation, dedifferentiation, and the progression of tumorigenicity.

Applications Tested: Flow Cytometry, Functional Studies (Blocking), Immunohistochemistry (Frozen, Paraffin), Immunoprecipitation, Western Blotting.

Target Information

Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.