Thermo Fisher Scientific Collagen I Monoclonal Antibody (NFI/20)

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

1:20

-

ELISA (ELISA)

1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

NFI/20

Immunogen

Human Collagen type I.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Ion-exchange chromatography, Ammonium sulfate precipitation

Storage buffer

PBS/0.01M sodium borate with 1% mannitol, 1% dextran

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_930061

Product Specific Information

MA1-83073 detects Collagen I in human sample types, and does not cross-react with collagen types II, III, or IV.

Reconstitute with 1 mL of distilled water. Prior to reconstitution, store at 4ºC. After reconstitution, store undiluted at -20ºC, avoiding freeze/thaw cycles.

Mouse anti Human Collagen I, clone NFI/20 recognizes human Collagen I composed of trimers consisting of 2 Collagen alpha-2(I) chains and a single Collagen alpha-1(I) chain forming collagen fibrils in ligaments, tendon, skin and bone where they become mineralized by calcium hydroxyapatite.

Target Information

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.