Thermo Fisher Scientific Complement C4d Monoclonal Antibody (10-11)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:750

-

Immunocytochemistry (ICC/IF)

1:250-1:600

-

ELISA (ELISA)

1:5,000-1:20,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

10-11

Immunogen

Native protein, from human plasma. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Complement C4d,uniProtId:P0C0L4-1,ncbiNodeId:9606,antigenRange:1-1744,antigenLength:1744,antigenImageFileName:MA1-83082_Complement_C4d_P0C0L4-1_House_mouse.svg,antigenImageFileNamePDP:MA1-83082_Complement_C4d_P0C0L4-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:Complement C4d,uniProtId:P0C0L5-1,ncbiNodeId:9606,antigenRange:1-1744,antigenLength:1744,antigenImageFileName:MA1-83082_Complement_C4d_P0C0L5-1_House_mouse.svg,antigenImageFileNamePDP:MA1-83082_Complement_C4d_P0C0L5-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Chromatography

Storage buffer

BBS, pH 8.4

Contains

<0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_928575

Product Specific Information

This antibody does not react with bovine, cat, dog, guinea pig, mouse, rabbit, rat or sheep.

It has been reported that this antibody works very well on acetone-fixed, frozen renal biopsies. Strong staining is observed in the glomeruli and in some cases the peritubular capillaries. Clone 10-11 has given variable results on formalin-fixed, paraffin-embedded sections. It has been observed that pre-treatment with 88% formic acid for 20 minutes at room temperature is beneficial.

Mouse anti Human C4d antibody, clone 10-11 recognizes the secreted protein complement component 4d (C4d).

Target Information

C4 is a component of the classical complement pathway and is split by activated C1s to C4a and C4b. Together C2a and C4b forms the classical C3 convertase. The molecular mass of C4 is 204 kDa and it consists of 3 subunits: a (97 kDa), b (75 kDa) and g (33 kDa).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.