Thermo Fisher Scientific CRF Monoclonal Antibody (5D5)
CRF 단백질을 인식하는 Mouse IgG1 단일클론 항체로 인간 시상하부 조직에서 검증됨. IHC(P) 및 ELISA에 사용 가능하며, 단백질 소화 및 항원 회수 과정 권장. 액상 형태로 1 mg/mL 농도, 4°C 또는 -20°C 보관. 연구용으로만 사용.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–10 µg/mL
- Publications: References
ELISA
- Tested Dilution: Assay-dependent
- Publications: References
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5D5 |
| Immunogen | Synthetic human corticotropin factor |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_930188 |
Product Specific Information
- Predicted to react with rat based on sequence homology.
- Requires protein digestion pre-treatment of paraffin sections using trypsin or pronase.
- Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for paraffin section staining.
- Suggested positive control: Human hypothalamus.
- Mouse anti-human corticotrophin-releasing factor antibody, clone 5D5, recognizes human corticotropin-releasing factor (CRF), a secreted hormone regulating corticotropin release from the pituitary.
Target Information
Corticotropin Releasing Factor (CRF) is a 41-amino-acid neuropeptide belonging to a family of structurally related peptides including urocortin, sauvagine, and urotensin I.
CRF plays a critical role in regulating the pituitary-adrenal axis and in endocrine, autonomic, and behavioral responses to stress.
It is widely distributed in the brain and peripheral nervous system, with the highest levels found in the hypothalamic paraventricular nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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