Thermo Fisher Scientific CRF Monoclonal Antibody (5D5)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1-10 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5D5

Immunogen

Synthetic human corticotropin factor

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_930188

Product Specific Information

Predicted to react with rat based on sequence homology.

This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for this product is human hypothalamus.

Mouse anti Human corticotrophin-releasing factor antibody, clone 5D5 recognizes human corticotropin-releasing factor (CRF), a secreted hormone which regulates the release of corticotropin from the pituitary.

Target Information

Corticotropin Releasing Factor (CRF), a 41 amino acids neuropeptide, belongs to a family of structurally related peptides including urocortin, sauvagine and urotensin I. CRF is critical in the regulation of the pituitary-adrenal gland axis, and in endocrine, autonomic and behavioral responses to stress. CRF is widely distributed in the brain and peripheral nervous system with the highest levels in the hypothalamic paraventricular nucleus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.