Thermo Fisher Scientific SaCas9 Monoclonal Antibody (26H10), GenCRISPR

Applications and Tested Dilution

Product Specifications

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) protein 9 system provides a robust and multiplexable genome editing tool, enabling researchers to precisely manipulate specific genomic elements. This facilitates the elucidation of target gene functions in biology and disease studies.

CRISPR/Cas9 genome editing allows for double-stranded DNA breaks at specific sequences to efficiently disrupt, excise, mutate, insert, or replace genes. The best characterized CRISPR-associated nucleases are the Cas9 proteins from Streptococcus pyogenes and Staphylococcus aureus. It is important that transfection precision and Cas9 expression levels are strictly controlled during editing processes using specific anti-CRISPR/Cas9 antibodies.

Target Information

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR adaptive immunity system in bacteria and genetic engineering. The system relies on RNA to target the nuclease to a desired DNA sequence, enabling indel mutations, specific sequence insertions, deletions, or genomic rearrangements at any desired location.

Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9).

SaCas9 has several advantages for genome editing, including small size, high efficiency, nickase activity, and high specificity. It recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9, with an ideal spacer length of 20–24 nucleotides. Compared to SpCas9, SaCas9 is smaller (1368 AA vs. 1053 AA) and generates indels at comparable rates, making it suitable for all-in-one delivery with adeno-associated vectors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.