Thermo Fisher Scientific c-Fos Monoclonal Antibody (2G9C3)

Applications

Tested Dilution

Publications

Western Blot (WB)

2.5-5 µg/mL

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2.5-5.0 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2G9C3

Immunogen

Synthetic peptide: KVEQLSPEEEEKRRIRRERNKMAAA, corresponding to Internal sequence amino acids 128 - 152 of Human c-Fos if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:c-Fos,uniProtId:P01100-1,ncbiNodeId:9606,antigenRange:128-152,antigenLength:380,antigenImageFileName:MA1-21190_c-Fos_P01100-1_House_mouse.svg,antigenImageFileNamePDP:MA1-21190_c-Fos_P01100-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

purified

Storage buffer

0.05M sodium phosphate, pH 7.5, with 0.2% gelatin

Contains

0.1% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_558385

Product Specific Information

This antibody does not cross-react with the 39 kDa jun protein.

By Western blot, this antibody detects ~55 & 62 kDa bands representing c-fos and v-fos, respectively. MA1-21190 is not suitable for IHC applications on frozen or paraffin tissues. Positive control of HeLa Cells suggested.

Target Information

FBJ murine osteosarcoma viral oncogene homolog is commonly known as Cellular protooncogene Fos (c-Fos). It belongs to the fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14. c-Fos heterodimerizes with c-Jun to form the Activator Protein -1 (AP-1) transcription factor which regulates transcription of several genes that play an important role in cellular signal transduction, cell proliferation and differentiation. c-Fos has been shown to be phosphorylated by various stimuli including growth factors and insulin at atleast seven different sites. The best known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK which regulates localization of c-Fos to the nucleus and is thus important for c-Fos induced transcriptional activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.