Thermo Fisher Scientific EGLN1/EGLN2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human EGLN1/EGLN2 (NP_0713341/NP_4442741) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PDH1/PDH2,uniProtId:Q96KS0-1,ncbiNodeId:9606,antigenRange:250-350,antigenLength:407,antigenImageFileName:PA5-109677_PDH1PDH2_Q96KS0-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109677_PDH1PDH2_Q96KS0-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:PDH1/PDH2,uniProtId:Q9GZT9-1,ncbiNodeId:9606,antigenRange:250-350,antigenLength:426,antigenImageFileName:PA5-109677_PDH1PDH2_Q9GZT9-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109677_PDH1PDH2_Q9GZT9-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.81 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2855088

Product Specific Information

Immunogen sequence: DIRGDKITWI EGKEPGCETI GLLMSSMDDL IRHCNGKLGS YKINGRTKAM VACYPGNGTG YVRHVDNPNG DGRCVTCIYY LNKDWDAKVS GGILRIFPEG K

Target Information

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.