Thermo Fisher Scientific Adenovirus Polyclonal Antibody, FITC

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:10-1:50

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Gradient purified, disrupted Adenovirus type 5 virions.

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

4-5 mg/mL

Purification

IgG fraction

Storage buffer

PBS, pH 7.2, with 10mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1016587

Product Specific Information

The PA1-73053 antibody reacts with Adenovirus types 1, 2, 3, 5, 6, 7a, 8, 31, 40 & 41. Others not tested. This antibody does not cross-react with Para 1-3, Influenza A &B, RSV, hMPV, and Rhinovirus (type 16). This antibody is negative against uninfected Hep-2, LLCMK2, and Vero cells by direct IF.

PA1-73053 has been successfully used in immunofluorescence applications. Direct immunofluorescence was performed on an acetone-fixed permissive tissue culture system

The PA1-73053 antibody was raised against gradient purified, disrupted Adenovirus type 5 virions.

Recommended short term storage of less than 6 months at 4ºC under subdued light. For long term storage, aliquot and store at -20ºC.

Target Information

Adenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). 49 human adenovirus serotypes have been discovered and divided into six subgroups, A to E, based on observation. They are an important cause of respiratory illness, conjunctivitis, gastroenteritis, and have more recently been recognized as a potential cause of sexually transmitted diseases. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterized by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated, causing disease. Adenovirus vectors, particularly those based on Ad5, have shown great promise for the development of gene therapy protocols and have been used for clinical purpose. Adenoviruses capsids have three principal protein components: the hexon, the penton, and the fiber. Hexon protein is a major coat protein (240 hexons per particle). Each hexon capsomere is homotrimer of the hexon protein, a complex protein of over 900 residues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.