Thermo Fisher Scientific beta Tubulin Polyclonal Antibody
Western blot, ICC/IF, IP 등 다양한 응용에 적합한 beta Tubulin 폴리클로날 항체. Rabbit IgG로 제작되었으며, Human, Mouse, Rat, Fish에 반응. 고순도(>95%) 항원 친화 크로마토그래피 정제. PBS/glycerol buffer, 4°C 또는 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fish, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant full-length Human Tubulin beta (UniProt ID: P04350-1, antigen range: 1–444) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2802872 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and is involved in neurogenesis, axon guidance, and maintenance. Mutations in the beta tubulin gene can cause congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin is also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
Glutamate residues at the C-terminus of beta III tubulin can be glutamylated, though the precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression, and this phosphorylation inhibits tubulin incorporation into microtubules.
Microtubules, the major cytoskeletal elements of eukaryotic cells, are composed of alpha and beta tubulin dimers (~55 kDa each) and play key roles in chromosome segregation, intracellular transport, ciliary and flagellar movement, and cytoskeletal structure. Beta-tubulin is widely used as a loading control in protein detection assays such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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