Thermo Fisher Scientific NFIB Polyclonal Antibody
NFIB 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, Flow Cytometry에 사용 가능. 인간 및 생쥐 시료에 반응하며, Protein A 정제 및 PBS 기반 버퍼에 보관. 연구용으로 DNA 결합 단백질 NF-1 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein within Human NFIB aa 1–273 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 50% Glycerol, 0.2% BSA |
| Contains | 0.05% Sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802866 |
Target Information
NF-1 (also designated CTF) consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 mRNA has revealed two forms of the NF-1 protein arising from alternative splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain and activates transcription through a proline-rich, carboxy-terminal transactivation domain. NF-1 recognizes and binds the adenovirus type 2 promoter and activates transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element is found in the upstream promoter region of various eukaryotic genes, including Ha-Ras, alpha-globin, HSP70, GRP78, Histone H1, myelin basic protein, and the Xenopus laevis vitellogenin gene promoter.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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