Thermo Fisher Scientific SPAM1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000
• Publications: -

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200
• Publications: -

Product Specifications

Product Specific Information

Antibody detects endogenous levels of total SPAM1. In mouse samples, this antibody also recognizes HYAL5 and HYAL2.

Target Information

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3.
This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1. Another family member on chromosome 3p21.3 has been assigned HYAL1.
This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, acts as a receptor in hyaluronic acid-induced cell signaling, and is involved in sperm-zona pellucida adhesion.
Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.