Thermo Fisher Scientific TWIST1 Monoclonal Antibody (2F8E7)
TWIST1 단백질을 인식하는 Mouse Monoclonal Antibody(2F8E7)로, Human 시료에 반응합니다. Western blot, ELISA, ICC/IF 등 다양한 응용에 적합하며, 고순도 Protein G 정제 및 안정적인 액상 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:200 | View publication |
| ELISA | 1:10,000 | View publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2F8E7 |
| Immunogen | Purified recombinant fragment of human TWIST1 (amino acids 9–74) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538666 |
Product Specific Information
MA5-17195 targets TWIST1 in indirect ELISA and WB applications, showing reactivity with Human samples.
The immunogen is a purified recombinant fragment of human TWIST1 (amino acids 9–74) expressed in E. coli.
MA5-17195 detects TWIST1, which has a predicted molecular weight of approximately 21 kDa.
Target Information
TWIST1 acts as a transcriptional regulator. It inhibits myogenesis by sequestering E proteins, suppresses trans-activation by MEF2, and blocks DNA-binding by MYOD1 through physical interaction.
TWIST1 also represses proinflammatory cytokines such as TNFA and IL1B, and regulates cranial suture patterning and fusion. Mutations in TWIST1 are implicated in Saethre-Chotzen syndrome, characterized by coronal synostosis, brachycephaly, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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