Thermo Fisher Scientific TWIST1 Monoclonal Antibody (2F8E7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

2F8E7

Immunogen

Purified recombinant fragment of human TWIST1 (amino acids: 9-74) expressed in E. Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TWIST1,uniProtId:Q15672-1,ncbiNodeId:9606,antigenRange:9-74,antigenLength:202,antigenImageFileName:MA5-17195_TWIST1_Q15672-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17195_TWIST1_Q15672-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538666

Product Specific Information

MA5-17195 targets TWIST1 in indirect ELISA, WB applications and shows reactivity with Human samples.

The MA5-17195 immunogen is purified recombinant fragment of human TWIST1 (amino acids: 9-74) expressed in E. Coli.

MA5-17195 detects TWIST1 which has a predicted molecular weight of approximately 21kDa.

Target Information

TWIST1 acts as a transcriptional regulator. It inhibits myogensis by sequestering E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. TWIST1 also represses proinflammatory cytokines such as TNFA and IL1B. TWIST also regulates cranial suture patterning and fusion and gene expression differentially depending on dimer composition. Mutations in the gene are implicated in Saethre-Chotzen syndrome, a disease characterized by coronal synostosis, brachycephaly, low front hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.