Thermo Fisher Scientific Ubiquitin B Monoclonal Antibody (3C12)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3C12

Immunogen

Purified recombinant fragment of human UBB expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538667

Product Specific Information

MA5-17196 targets UBB in FACS, indirect ELISA, and WB applications and shows reactivity with Human samples.

The MA5-17196 immunogen is purified recombinant fragment of human UBB expressed in E. Coli.

MA5-17196 detects UBB which has a predicted molecular weight of approximately 26kDa.

Target Information

Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimers disease, Lewy bodies seen in Parkinsons disease, and Pick bodies found in Pick`s disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.