Thermo Fisher Scientific LAP (Latency Associated Peptide) Monoclonal Antibody (7F6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7F6

Immunogen

Purified recombinant fragment of human TGFb1 (amino acids: 62-195) expressed in E. Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LAP,uniProtId:P01137-1,ncbiNodeId:9606,antigenRange:62-195,antigenLength:390,antigenImageFileName:MA5-17186_LAP_P01137-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17186_LAP_P01137-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538657

Product Specific Information

MA5-17186 targets TGFb1 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human samples.

The MA5-17186 immunogen is purified recombinant fragment of human TGFb1 (amino acids: 62-195) expressed in E. Coli.

MA5-17186 detects TGFb1 which has a predicted molecular weight of approximately 44.3kDa.

Target Information

TGF beta-1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its prodomain. Activation of TGF-b1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF-beta, an aminoterminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.