Thermo Fisher Scientific Hemoglobin alpha Recombinant Rabbit Monoclonal Antibody (PD00-16)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Positive control: Rat heart tissue lysates, human bone marrow tissue, human liver tissue, human placenta tissue, mouse brain tissue, human spleen tissue
• Predicted band size: 15 kDa
• Subcellular Location: Cytosol, cytosolic small ribosomal subunit, hemoglobin complex, blood microparticle, extracellular exosome, extracellular region, extracellular space, endocytic vesicle lumen, haptoglobin-hemoglobin complex, membrane

Target Information

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5′- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3′.
The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical, differing slightly in untranslated and intronic regions but significantly in 3′ untranslated regions.
Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of total hemoglobin; alpha chains combine with delta chains to form HbA-2, which along with HbF (fetal hemoglobin) makes up the remaining 3%.
Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.