Thermo Fisher Scientific Hemoglobin alpha Recombinant Rabbit Monoclonal Antibody (PD00-16)
Hemoglobin alpha 단백질을 인식하는 Rabbit Recombinant Monoclonal Antibody. Western blot 및 IHC(P) 검증 완료. Human, Mouse, Rat 반응성. Protein A 정제, 1 mg/mL 농도, PBS buffer에 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:4,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PD00-16 |
| Immunogen | Synthetic peptide corresponding to Human Hemoglobin subunit alpha aa 50–150/142 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3094238 |
Product Specific Information
- Positive control: Rat heart tissue lysates, human bone marrow tissue, human liver tissue, human placenta tissue, mouse brain tissue, human spleen tissue
- Predicted band size: 15 kDa
- Subcellular Location: Cytosol, cytosolic small ribosomal subunit, hemoglobin complex, blood microparticle, extracellular exosome, extracellular region, extracellular space, endocytic vesicle lumen, haptoglobin-hemoglobin complex, membrane
Target Information
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5′- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3′.
The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical, differing slightly in untranslated and intronic regions but significantly in 3′ untranslated regions.
Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of total hemoglobin; alpha chains combine with delta chains to form HbA-2, which along with HbF (fetal hemoglobin) makes up the remaining 3%.
Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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