Thermo Fisher Scientific Phospho-PKC mu (Tyr463) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (IHC)

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PRKD1(Accession Q15139), corresponding to amino acid residues around phosphorylated Tyr463. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PKC mu,uniProtId:Q15139-1,ncbiNodeId:9606,antigenRange:463,antigenLength:912,antigenImageFileName:PA5-104995_PKC_mu_Q15139-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104995_PKC_mu_Q15139-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:PKC mu,uniProtId:O94806-1,ncbiNodeId:9606,antigenRange:157,antigenLength:890,antigenImageFileName:PA5-104995_PKC_mu_O94806-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104995_PKC_mu_O94806-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816468

Product Specific Information

Antibody detects endogenous levels of PKD1/PKC mu only when phosphorylated at Tyrosine 463.

Target Information

The PKC family of serine/threonine kinases, including PRKCN (PKD3), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. PKC mu activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.