Thermo Fisher Scientific Phospho-PKD1/2/3/PKC mu (Ser738, Ser742) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PRKD1(Accession Q15139), corresponding to amino acid residues around phosphorylated Ser738. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PKD1/2/3/PKC mu,uniProtId:Q15139-1,ncbiNodeId:9606,antigenRange:738,antigenLength:912,antigenImageFileName:PA5-104996_PKD123PKC_mu_Q15139-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104996_PKD123PKC_mu_Q15139-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:PKD1/2/3/PKC mu,uniProtId:Q9BZL6-1,ncbiNodeId:9606,antigenRange:736|744,antigenLength:878,antigenImageFileName:PA5-104996_PKD123PKC_mu_Q9BZL6-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104996_PKD123PKC_mu_Q9BZL6-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:PKD1/2/3/PKC mu,uniProtId:O94806-1,ncbiNodeId:9606,antigenRange:735|738,antigenLength:890,antigenImageFileName:PA5-104996_PKD123PKC_mu_O94806-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104996_PKD123PKC_mu_O94806-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816469

Product Specific Information

Antibody detects endogenous levels of PKD1/2/3/PKC mu only when phosphorylated at Serine 738+Serine 742.

Target Information

Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of MAPK8/JNK1 and Ras signaling, Golgi membrane integrity and trafficking, cell survival through NF-kappa-B activation, cell migration, cell differentiation by mediating HDAC7 nuclear export, cell proliferation via MAPK1/3 (ERK1/2) signaling, and plays a role in cardiac hypertrophy, VEGFA-induced angiogenesis, genotoxic-induced apoptosis and flagellin-stimulated inflammatory response. Phosphorylates the epidermal growth factor receptor (EGFR) on dual threonine residues, which leads to the suppression of epidermal growth factor (EGF)-induced MAPK8/JNK1 activation and subsequent JUN phosphorylation. Phosphorylates RIN1, inducing RIN1 binding to 14-3-3 proteins YWHAB, YWHAE and YWHAZ and increased competition with RAF1 for binding to GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). Acts downstream of the heterotrimeric G-protein beta/gamma-subunit complex to maintain the structural integrity of the Golgi membranes, and is required for protein transport along the secretory pathway. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.