Thermo Fisher Scientific Phospho-4EBP1 (Thr37, Thr46) Recombinant Rabbit Monoclonal Antibody (4EB1T37T46-A5)
Phospho-4EBP1 (Thr37, Thr46) 항체로 인간, 마우스, 랫트 시료에 반응합니다. Western blot, IHC, Flow cytometry에 적합하며, 재조합 토끼 모노클론 항체로 높은 특이성과 일관성을 제공합니다. 단백질 번역 조절 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | Assay-dependent |
| Flow Cytometry (Flow) | 1–1,000 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4EB1T37T46-A5 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Thr37/46 of human phospho 4E-BP1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 50% glycerol, 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745012 |
Available Conjugated Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
This gene encodes a member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is crucial for recruiting 40S ribosomal subunits to the 5′ end of mRNAs.
Binding of this protein to eIF4E inhibits complex assembly and represses translation. Phosphorylation in response to signals such as UV irradiation and insulin signaling causes dissociation from eIF4E, thereby activating mRNA translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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