Thermo Fisher Scientific Phospho-Histone H2A.X (Ser139) Recombinant Rabbit Monoclonal Antibody (HisH2AXS139-1E4)
Phospho-Histone H2A.X (Ser139)을 인식하는 recombinant rabbit monoclonal antibody로, Western blot 및 Flow cytometry에 적합합니다. 높은 특이성과 재현성을 제공하며, lot 간 일관성이 우수합니다. PBS buffer 기반의 액상 형태로 -20°C에서 보관합니다.
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Applications
Western Blot (WB)
- Assay-dependent
Flow Cytometry (Flow)
- 1–1,000 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HisH2AXS139-1E4 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser139 of human phospho histone H2A.X |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745014 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened for optimal production. Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal-origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Histone H2A.X (H2AX) is a member of the histone H2A family forming part of the nucleosome core particle. Upon DNA double-strand breaks (DSBs), phosphorylation occurs at Ser139, generating gamma-H2AX. This modification can be detected by Western blotting or immunofluorescence to assess DSB frequency.
Phosphorylation may involve kinases such as ATM, DNA-PK, and ATR. H2A.X consists of 143 amino acids and contributes to chromatin structure and DNA repair mechanisms. It maps to the 11q23.2–q23.3 region of the human chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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