Thermo Fisher Scientific Phospho-EGFR (Tyr1068) Recombinant Rabbit Monoclonal Antibody (EGFRY1068-E5), PE
Phospho-EGFR (Tyr1068) 인산화 부위를 인식하는 재조합 토끼 단일클론 항체로, PE 형광 표지되어 유세포분석(Flow Cytometry)에 최적화됨. 높은 특이성과 재현성을 제공하며, 인간, 마우스, 랫트 시료에 반응. 단백질 A/G 정제, 4°C 보관.
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Applications
- Flow Cytometry (Flow): 5 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | EGFRY1068-E5 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human phospho EGFR |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745010 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed, and individual clones are screened for optimal performance.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broad immunoreactivity due to the large rabbit immune repertoire
Target Information
EGFR (Epidermal Growth Factor Receptor) is a receptor tyrosine kinase that mediates signaling in response to various growth factors. Overexpression of EGFR has been associated with multiple cancers and serves as a therapeutic target for biological and small molecule inhibitors.
The EGFR gene is located on chromosome 7 in humans and belongs to the HER/ERbB family, which also includes ERbB2, ERbB3, and ERbB4.
Ligand binding (e.g., EGF, TGF-α) induces receptor dimerization and autophosphorylation of tyrosine residues, triggering downstream signaling pathways.
Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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