Thermo Fisher Scientific CD243 (ABCB1) Monoclonal Antibody (UIC2)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Flow Cytometry (Flow)

1-4 µg/mL

-

Immunoprecipitation (IP)

Assay-Dependent

-

Functional Assay (Functional)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

UIC2

Immunogen

Human CD243 (MDR-1) cDNA- transfected NIH 3T3 cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:P-Glycoprotein (ABCB1),uniProtId:P08183-1,ncbiNodeId:9606,antigenRange:1-1280,antigenLength:1280,antigenImageFileName:MA5-28587_P-Glycoprotein_ABCB1_P08183-1_House_mouse.svg,antigenImageFileNamePDP:MA5-28587_P-Glycoprotein_ABCB1_P08183-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2745546

Product Specific Information

This antibody recognizes an extracellular epitope on CD243 (MDR-1), an approximately 170 kDa ABC transporter expressed on hematopoietic stem cells, B, T, and NK cells, or on many multidrug resistant cancer cells.

This antibody preferentially recognizes CD243 in the process of transporting substrate. Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 µm filter sterilized.

Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunohistochemistry (Paraffin): Heat mediated antigen retrieval in citrate buffer pH 6; Functional Application: Inhibition.

Target Information

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.