Thermo Fisher Scientific CD87 (UPAR) Monoclonal Antibody (VIM5), PE

Applications

Tested Dilution

Publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Flow Cytometry (Flow)

10 µL/1x10^6 cells

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Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

VIM5

Immunogen

human myeloid cell line THP-1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:uPAR (CD87),uniProtId:Q03405-1,ncbiNodeId:9606,antigenRange:1-335,antigenLength:335,antigenImageFileName:MA5-28588_uPAR_CD87_Q03405-1_House_mouse.svg,antigenImageFileNamePDP:MA5-28588_uPAR_CD87_Q03405-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2745547

Product Specific Information

This antibody recognizes CD87 (urokinase plasminogen activator receptor), a 36-68 kDa single-chain GPI-anchored extracellular glycoprotein expressed on granulocytes, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts and keratinocytes.

Recommended dilution is for 100 µL of whole blood or 1x10^6 cells in suspension.

Target Information

The urokinase-type plasminogen activator receptor is a key molecule in the regulation of cell-surface plasminogen activation and plays an important role in many normal as well as pathologic processes. The human PLAUR cDNA encodes 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. It produces a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. PLAUR, also known as UPAR, is directly associated with the carbohydrate-binding domain of SELL in the membrane of neutrophils, an association analogous to that between PLAUR and beta-2 integrins. PLAUR-mediated calcium mobilization is SELL dependent. UPAR mRNA levels correlate with the invasive potential of endometrial carcinomas and show a 33-fold increase in UPAR mRNA levels in advanced clinical stage endometrial tumors compared with normal endometrial tissue. Furthermore, the increase in UPAR mRNA levels correlated linearly with the progression of disease stage. UPAR protein expressioin correlated positively with rate of recurrence and mortality in patients with endometrial cancer. UPAR appears to be a useful prognostic marker for advanced endometrial cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.