Thermo Fisher Scientific B7-2 (CD86) Monoclonal Antibody (OTI9D1)
Human CD86 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 Flow cytometry에 적합합니다. 동결건조 형태로 제공되며 PBS와 trehalose로 안정화되어 있습니다. 항원은 HEK293T 세포에서 발현된 full-length human CD86 단백질입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9D1 |
| Immunogen | Full length human recombinant protein of human CD86 (NP_787058) produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CD86 is one of two ligands (the other being CD80) for CTLA4 and CD28. It functions as a costimulatory molecule in T-cell activation during antigen presentation.
Insufficient co-stimulation via CD86/CD80 can lead to immune tolerance.
CD86 is a type I membrane protein belonging to the immunoglobulin superfamily and is expressed by antigen-presenting cells.
It binds to CD28 antigen and cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) on T cells, providing stimulatory or inhibitory signals, respectively.
Alternative splicing produces multiple transcript variants.
Dysfunction of CD86 has been associated with gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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