Thermo Fisher Scientific IL1RA Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific IL1RA Polyclonal Antibody, Biotin (PeproTech)

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-1RA. Anti-Human IL-1RA-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human IL-1RA by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-1RA (500-P209) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human IL-1RA.

Western Blot:
To detect Human IL-1RA by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human IL-1RA is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P209BT-1MG will be provided as 2 × 500 µg.

Target Information

The protein encoded by this gene is a cytokine receptor belonging to the interleukin 1 receptor family. It acts as a receptor for IL1A, IL1B, and IL1R1/IL1RA, and plays a key role in cytokine-induced immune and inflammatory responses. This gene, along with IL1R2, IL1RL2, and IL1RL1, forms a cytokine receptor gene cluster mapped to chromosome 2q12.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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