Thermo Fisher Scientific IL-10 Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Rabbit Polyclonal Anti-Human IL-10 항체로 ELISA 및 Western Blot에 적합. 높은 특이성과 민감도로 IL-10 단백질 검출 가능. 항원 친화 크로마토그래피로 정제되어 신뢰성 높은 연구 결과 제공.
- 카탈로그번호
- 500-P20BT-500UG
- 판매단위
- pk
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Thermo Fisher Scientific IL-10 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IL-10 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
AA Sequence of recombinant protein:
MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-10. Anti-Human IL-10-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect human IL-10 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-10 (500-P20) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hIL-10.
Western Blot:
To detect hIL-10 by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. In conjunction with compatible secondary reagents, the detection limit for Recombinant hIL-10 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Packaging:
500-P20BT-1MG will be provided as 2 × 500 µg.
Target Information
IL-10 encodes a cytokine primarily produced by monocytes, with some production by lymphocytes. It plays key roles in immunoregulation and inflammation, including:
- Down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages
- Enhancing B cell survival, proliferation, and antibody production
- Blocking NF-kappa B activity
- Regulating the JAK-STAT signaling pathway
Knockout studies in mice indicate IL-10 is essential for intestinal immune regulation. Mutations in IL10 are associated with increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases related to IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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