Thermo Fisher Scientific Galectin 1 Polyclonal Antibody, PeproTech
Rabbit polyclonal antibody against Human Galectin-1. Validated for WB, IHC, ICC, ELISA, and in vitro assays. High specificity and sensitivity with antigen affinity purification. Suitable for research use in inflammation and immune regulation studies.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 3 publications |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunocytochemistry (ICC/IF) | – | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 2 publications |
| In vitro Assay (IV) | – | View 2 publications |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
| Immunostaining (IS) | – | View 3 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Chicken, Hamster, Human, Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human Galectin-1 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929825 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Galectin-1. The antibody was purified by affinity chromatography using an immobilized Human Galectin-1 matrix.
Sandwich ELISA:
For detection of Human Galectin-1 by sandwich ELISA, use 0.5–2.0 µg/mL antibody (100 µL/well). In combination with PeproTech Biotinylated Anti-Human Galectin-1 (500-P210Bt), the detection limit is 0.2–0.4 ng/well of Recombinant Human Galectin-1.
Western Blot:
For Western Blot analysis, use 0.1–0.2 µg/mL antibody. Detection limit for Recombinant Human Galectin-1 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
Galectin-1 is a member of the lectin family of carbohydrate-binding proteins. It exists in monomeric or homodimeric forms and is expressed in various cell and tissue types. Galectin-1 regulates cell growth, proliferation, and induces apoptosis in activated T cells. It also modulates cytokine secretion and inhibits pro-inflammatory cytokine production, playing a key role in acute and chronic inflammation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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