Thermo Fisher Scientific NOX4 Recombinant Rabbit Monoclonal Antibody (SY0214)
NOX4 단백질을 인식하는 재조합 토끼 단클론 항체로, 높은 특이성과 민감도를 제공합니다. Western blot, IHC, ICC 등 다양한 응용에 사용 가능하며, 로트 간 일관성과 동물 유래 성분이 없는 포뮬레이션을 특징으로 합니다. 연구용 전용 제품입니다.
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Thermo Fisher Scientific NOX4 Recombinant Rabbit Monoclonal Antibody (SY0214)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | View 7 publications |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Functional Assay (Functional) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SY0214 |
| Immunogen | Recombinant protein within Human NOX4 aa 460–578 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809383 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
Target Information
Oxygen sensing is essential for homeostasis in all aerobic organisms. A phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes, has been postulated to function in the kidney as an oxygen sensor regulating the synthesis of erythropoietin in the renal cortex.
NOX4 acts as a redox messenger involved in intracellular signaling pathways contributing to mitochondriogenesis, cell survival, and differentiation in hematopoietic stem cells. Data suggest that NOX4 provides a link between the insulin receptor and the generation of cellular ROS, enhancing insulin signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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