Thermo Fisher Scientific SMAD3 Recombinant Rabbit Monoclonal Antibody (SY25-01)
SMAD3 단백질을 인식하는 재조합 토끼 모노클로날 항체로 높은 특이성과 민감도를 제공합니다. Western blot, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합합니다. HEK293 세포에서 발현되었으며, 일관된 로트 간 재현성과 동물 유래 성분이 없는 포뮬레이션을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:500 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SY25-01 |
| Immunogen | Synthetic peptide within Human Smad3 aa 181–230 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809384 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences are cloned from immunoreactive rabbits and screened to select optimal production clones.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to large rabbit immune repertoire
Target Information
SMAD3 belongs to the SMAD protein family, homologous to the Drosophila mothers against decapentaplegic (Mad) and C. elegans Sma genes.
SMAD proteins act as signal transducers and transcriptional modulators mediating multiple signaling pathways.
SMAD3 is activated by transforming growth factor-beta (TGF-β) and plays a role in transcriptional regulation and carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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