Thermo Fisher Scientific Phospho-STAT3 (Ser727) Recombinant Rabbit Monoclonal Antibody (SY24-09)
Phospho-STAT3 (Ser727) 단백질을 인식하는 재조합 토끼 단클론 항체. Western blot, IHC, ICC/IF에 적합하며, 높은 특이성과 일관된 로트 간 재현성을 제공. Human, Mouse, Rat 시료에 반응하며, Protein A 정제 및 동물 유래 성분 무첨가 포뮬레이션.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SY24-09 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser727 of Human STAT3 (aa 701–750) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809382 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select the best candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal-origin free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
STAT3 (Signal Transducer and Activator of Transcription 3) is a transcription factor activated by cytokines and growth factors. It is phosphorylated by receptor-associated kinases, translocates to the nucleus, and regulates gene expression.
- Activation by: IFN-α, IFN-γ, EGF, PDGF, IL-6
- Phosphorylation at Tyr705 by JAK1/JAK2 is essential for dimerization and DNA binding
- Located on chromosome 17q in humans
- Plays a key role in cell growth, apoptosis, and transcriptional regulation
- Interacts with coactivators such as CREB-binding protein and transcription factors c-Jun and CREB
- PIAS3 acts as a specific inhibitor of STAT3
- Three alternatively spliced transcript variants exist
- Knockout of STAT3 in mice is embryonically lethal
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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