Thermo Fisher Scientific CD273 (B7-DC) Monoclonal Antibody (TY25), eBioscience
Mouse B7-DC/PD-L2 인식하는 TY25 단클론 항체로, Flow Cytometry, WB, IHC 등 다양한 응용 가능. PD-1 결합 연구 및 APC/T cell 상호작용 분석에 유용. 비환원 조건에서 사용 권장하며, 0.5 µg/test로 최적화됨.
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Thermo Fisher Scientific CD273 (B7-DC) Monoclonal Antibody (TY25), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | 17 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | Assay-Dependent | - |
| Functional Assay (Functional) | Assay-Dependent | 2 publications |
| Inhibition Assays (IA) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Hamster, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TY25 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467788 |
Additional Formats
Product Specific Information
The TY25 monoclonal antibody reacts with mouse B7-DC/PD-L2, a member of the B7 family (~25 kDa). B7-DC is expressed mainly by dendritic cells, monocytes, and macrophages. It binds PD-1 but not CD28/CTLA-4, mediating costimulation or suppression of T cell proliferation depending on activation state. TY25 is useful for studying B7-DC/PD-L2 function and expression in APC/T cell interactions.
Applications Reported:
TY25 has been used for flow cytometry, immunoprecipitation, Western blot (non-reducing conditions), and IHC of frozen sections. Also reported for ligand inhibition assays. Functional assays should use Functional Grade purified TY25 (Product #16-5986).
Applications Tested:
Tested by flow cytometry on mouse splenocytes and B7-DC transfected cells. Recommended ≤0.5 µg/test in 100 µL final volume. Cell number: 10⁵–10⁸ cells/test. Titrate for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Programmed death-ligand 2 (PD-L2, B7-DC) is part of the B7 ligand family and binds PD-1. Expressed mainly in dendritic cells but inducible in other immune and non-immune cells. Expression is upregulated by IL-4 and TNF-α/IFN-γ. PD-L2 shows higher affinity to PD-1 than PD-L1 and functions as an inhibitory immune checkpoint molecule. It can also stimulate T cell proliferation even in PD-1-deficient cells, suggesting additional receptors such as RGMb. PD-L2–RGMb interaction regulates respiratory tolerance via BMP/neogenin signaling.
Human PD-L2 monomer:
- Extracellular domain: 201 aa
- Transmembrane domain: 21 aa
- Cytoplasmic domain: 32 aa
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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