Thermo Fisher Scientific Nur77 Monoclonal Antibody (12.14), eBioscience
Mouse Nur77 단백질을 인식하는 12.14 클론 단일클론 항체로, Western blot에서 검증됨. NR4A1 관련 세포사멸 및 신호전달 연구에 적합. 순도 90% 이상, 액상 형태로 4°C 보관. 다양한 형광 및 효소 결합 형식으로 제공 가능.
- 카탈로그번호
- 14-5965-xx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Nur77 Monoclonal Antibody (12.14), eBioscience
Thermo Fisher Scientific Nur77 Monoclonal Antibody (12.14), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 5 µg/mL | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | - | View 11 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12.14 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865185 |
Available Conjugated Formats
- Alexa Fluor 488
- Alexa Fluor 647
- eFluor 450
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
Description:
The 12.14 monoclonal antibody reacts with mouse Nur77 (NR4A1, TR3, NGFI-B, NAK1), an inducible orphan nuclear receptor. Nur77 is expressed in thymocytes and T cell lines, promoting apoptosis and playing a role in thymocyte negative selection. It is also critical for steroid biosynthesis in Leydig cells and dopamine signaling. Nur77 interacts with FoxP3 in regulatory T cells, though results with this antibody do not correlate with that observation.
Applications Reported:
This antibody has been reported for use in immunoblotting (WB).
Applications Tested:
Tested by Western blot on mouse thymocyte lysates stimulated with PMA and ionomycin for 2 hours. Recommended concentration: 5 µg/mL (titrate for optimal performance).
Purity:
90%, determined by SDS-PAGE.
Aggregation:
<10%, determined by HPLC.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. It is expressed in brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung, and prostate. Expression is induced by stress stimuli and growth factors. Acts as a nuclear transcription factor mediating apoptotic signaling in thymocytes and tumor cells, and functions in hypothalamic-pituitary axis signaling. Translocation from nucleus to mitochondria induces apoptosis.
Three transcript variants encode two distinct isoforms of NR4A1. Variant (3), also known as TRC beta, has multiple differences from variant 1, resulting in isoform b with a shorter and distinct C-terminus. RXR is a known partner for NR4A1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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