Thermo Fisher Scientific Nur77 Monoclonal Antibody (12.14), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 11 publications 11 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

12.14

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 647
• eFluor 450
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865185

Product Specific Information

Description: This 12.14 monoclonal antibody reacts with mouse Nur77 (also known as NR4A1, TR3, NGFI-B, or NAK1), an inducible orphan nuclear receptor. Expressed in thymocytes and T cell lines, Nur77 promotes apoptosis and plays a role in thymocyte negative selection. Additionally, Nur77 has been shown to be critical for steroid biosynthesis in Leydig cells as well as for the effects of dopamine. In addition, Nur77 has been shown to interact with FoxP3 in regulatory T cells. However, our results with this antibody do not correlate with this observation.

Applications Reported: This 12.14 antibody has been reported for use in immunoblotting (WB).

Applications Tested: This 12.14 antibody has been tested by western blot on lysates prepared from mouse thymocytes stimulated with PMA and ionomycin for two hours. This antibody can be used at 5 µg/mL. It is recommended that this antibody by titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. NGFI-B-alpha is expressed in brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung and prostate. Its expression is induced in response to various stress stimuli and growth factors. The encoded protein acts as a nuclear transcription factor involved in mediating apoptotic signaling in thymocytes and tumor cells, as well as having a role in signaling in the hypothalamic-pituitary axis. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Three transcript variants encoding two distinct isoforms have been identified for this NR4A1. Variant (3), also known as TRC beta, contains multiple differences compared to variant 1. The resulting isoform b contains a shorter and distinct C-terminus compared to isoform a. RXR has been shown to be a partner for NR4A1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.