Thermo Fisher Scientific MHC Class II (I-Ek) Monoclonal Antibody (14-4-4S), eBioscience

Thermo Fisher Scientific MHC Class II (I-Ek) Monoclonal Antibody (14-4-4S), eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• FITC Conjugate
• PE Conjugate
• Request custom conjugation

Product Specific Information

The 14-4-4S monoclonal antibody reacts with mouse and rat MHC class II I-E subregion glycoprotein in strains of H-2^k, H-2^d, H-2^p, and H-2^r. Haplotypes H-2^b, H-2^f, and H-2^s do not express the I-E antigen.
MHC class II is present on B cells, monocytes, macrophages, and dendritic cells. The 14-4-4S mAb has been reported to block antigen presentation in vitro. This antibody cross-reacts with rat.

Applications Reported: Flow cytometry, immunoprecipitation, complement-dependent cytotoxicity, and IHC (frozen).
(Biotinylated 14-4-4S, Product #13-5980, recommended for IHC.)

Applications Tested: Tested by flow cytometric analysis of mouse splenocytes; usable at ≤0.5 µg/test in 100 µL final volume. Cell number range: 10^5–10^8 cells/test. Antibody titration recommended for optimal performance.

Target Information

MHC class II molecules are transmembrane glycoproteins expressed on professional antigen-presenting cells (macrophages, dendritic cells, B cells). They bind endocytosed exogenous antigens and present them to T cells.
The antigen-binding groove between MHC class II α and β chains is open at both ends and 15–24 amino acids long.

HLA and MHC antibodies are essential in immunopeptidomics, aiding neoantigen identification and characterization via LC–MS/MS.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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