Thermo Fisher Scientific Human Fc gamma RIIA (CD32a) (167 His) His-tag Recombinant Protein
인간 Fc gamma RIIA (CD32a) His-tag 재조합 단백질로 다양한 면역 분석(WB, IHC, ELISA, SPR)에 사용 가능. HEK293 세포에서 발현되었으며, 고순도(>95%)와 낮은 엔도톡신(<1.0 EU/µg)을 특징으로 함. 항체 결합 활성 검증 완료.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Surface Plasmon Resonance (SPR): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | HEK293 cells |
| Amino Acid Sequence | Human FCGR2A, amino acids Met1–Ile218 (Accession # P12318-1) with a C-terminal His-tag |
| Tag | His-tag |
| Molecular Weight | 22 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% (SDS-PAGE), >90% (SEC-HPLC) |
| Endotoxin Concentration | <1.0 EU/µg |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient |
Activity
- Loaded Rituximab (IgG1) on ProA Biosensor: binds Human Fc gamma RIIA (CD32a) His-tag Recombinant Protein with affinity constant 1.7 µM (BLI assay, Sartorius Octet Red384, routinely tested).
- Loaded Human Fc gamma RIIA (CD32a) His-tag Recombinant Protein on His1K Biosensor: binds Bevacizumab (IgG1) with affinity constant 0.55 µM (BLI assay, Sartorius Octet Red384, QC tested).
- Captured Bevacizumab (IgG1) on ProA Chip: binds Human Fc gamma RIIA (CD32a) His-tag Recombinant Protein with affinity constant 0.58 µM (SPR assay, Biacore T200, routinely tested).
Product Specific Information
Reconstitution:
Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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