Thermo Fisher Scientific Human Fc gamma RIIA (CD32a) (167 Arg/His) His-tag Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Surface plasmon resonance (SPR)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

HEK293 cells

Amino acid sequence

Human FCGR2A, amino acids Met1-Ile218 (Accession # P12318-1) with natural variant H167R substitution with a C-terminal His-tag

Tag

His-tag

Molecular weight

22 kDa

Class

Recombinant

Type

Protein

Purity

>95 % as determined by SDS-PAGE. >90 % as determined by SEC-HPLC

Endotoxin concentration

<1.0 EU/µg

Activity

1.Loaded Rituximab (IgG1) on ProA Biosensor, can bind Human Fc gamma RIIA (CD32a) (167 Arg/His) His-tag Recombinant Protein with an affinity constant of 2.3 µM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested).
2.Loaded Human Fc gamma RIIA (CD32a) (167 Arg/His) His-tag Recombinant Protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.65 µM as determined in a BLI assay (Sartorius Octet Red384) (QC tested).
3.Captured Bevacizumab (IgG1) on proA Chip can bind Human Fc gamma RIIA (CD32a) (167 Arg/His) His-tag Recombinant Protein with an affinity constant of 0.79 µM as determined in an SPR assay (Biacore T200) (Routinely tested).
4.The purity of Human Fc gamma RIIA (CD32a) (167 Arg/His) His-tag Recombinant Protein is more than 95% and the molecular weight of this protein is around 23-32 kDa verified by SEC-MALS.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient

Product Specific Information

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Target Information

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.