Thermo Fisher Scientific Human IL-13 Recombinant Protein
인간 IL-13 재조합 단백질로 면역 및 염증 반응 조절 연구에 사용됩니다. CHO 세포에서 발현되며 92% 이상 순도, 낮은 엔도톡신 수준을 가집니다. 세포 증식 분석에서 기능적 활성을 검증하였으며, WB, IHC, ELISA 등에 적용 가능합니다.
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Thermo Fisher Scientific Human IL-13 Recombinant Protein
Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA (ELISA): Assay-dependent
- Functional Assay (Functional): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | CHO cells |
| Amino Acid Sequence | Human IL-13, amino acids Met1–Asn132 (Accession # AAK53823.1) |
| Molecular Weight | 12.5 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >92% by SDS-PAGE |
| Endotoxin Concentration | <1.0 EU/µg |
| Activity | Measured in a cell proliferation assay using TF1 human erythroleukemic cells. ED50 typically 0.5–3 ng/mL |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient |
Product Specific Information
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses.
It down-regulates macrophage activity, inhibiting production of pro-inflammatory cytokines and chemokines, and plays a role in allergen-induced asthma through mechanisms independent of IgE and eosinophils.
IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells.
Structurally, IL-13 closely resembles IL-3, IL-4, IL-5, and GM-CSF. The genes encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with IL-13 located near IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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