Thermo Fisher Scientific Human ICAM-1 (CD54) His-tag Recombinant Protein
Human ICAM-1 (CD54) 재조합 단백질로, HEK293 세포에서 발현된 His-tag 단백질입니다. 세포 부착 기능 검증 완료, ≥98% 고순도, ELISA·WB·IHC 등 다양한 연구 응용 가능. 동결건조 형태로 제공되며, 연구용으로만 사용됩니다.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | HEK293 cells |
| Amino Acid Sequence | Human ICAM1, amino acids Met1–Glu480 (Accession # NP_000192.2) with a C-terminal His-tag |
| Tag | His-tag |
| Molecular Weight | 51 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥98% (SDS-PAGE), ≥90% (SEC-HPLC) |
| Endotoxin Concentration | <1.0 EU/µg |
| Activity | Supports adhesion of PMA-stimulated HSB2 cells; 30–60% adhere to ICAM1-coated plates (12.5 µg/mL, 100 µL/well) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS (pH 7.4), 5% mannitol, 5% trehalose, 0.01% Tween 80 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C (preferred) |
| Shipping Conditions | Ambient |
Product Specific Information
Reconstitution:
Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended to aliquot and use immediately. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein with an extracellular domain containing five immunoglobulin superfamily repeats, a transmembrane region, and a cytoplasmic domain.
It has seven potential N-linked glycosylation sites and shares sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102).
ICAM-1 binds to integrins CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1), and serves as a receptor for Rhinovirus.
Expression occurs in activated endothelial cells, epithelial cells, fibroblasts, chondrocytes, B and T lymphocytes, monocytes, macrophages, dendritic cells, and neutrophils, with levels increasing upon inflammation.
Detected also in certain carcinoma and melanoma cells. Soluble ICAM-1 is found in plasma and elevated in inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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