Thermo Fisher Scientific Human PD-1 His-tag Fc Chimera Recombinant Protein
Human PD-1 단백질을 HEK293 세포에서 발현한 재조합 단백질로, Fc 및 His 태그를 포함합니다. ELISA, Western blot, IHC 등 다양한 면역학적 분석에 사용됩니다. 고순도(>97%)이며, PD-L2와의 결합 활성을 검증받았습니다. 연구용으로만 사용 가능합니다.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | HEK293 cells |
| Amino Acid Sequence | Human PD-1, amino acids Met1–Gln167 (Accession #NP_005009.2) with a C-terminal His-tagged human IgG1 Fc-tag |
| Tag | Fc-tag, His-tag |
| Molecular Weight | 44 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >97% by SDS-PAGE |
| Endotoxin Concentration | <1.0 EU/µg |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized recombinant human PD-L2 at 1 µg/mL (100 µL/well) can bind human PD-1/Fc chimera with a linear range of 7.8–1000 ng/mL. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient |
Product Specific Information
Reconstitution:
Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
Cell-mediated immune responses are initiated by T lymphocytes that are stimulated by peptides bound to MHC molecules on antigen-presenting cells (APC).
Activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from APCs. PD-1 binds to two ligands, PD-L1 and PD-L2.
Upon ligand binding, PD-1 inhibits immune activation in the absence of “danger signals” such as LPS or other pathogen-associated molecules.
PD-1 deficiency in mice leads to hyperactivated immune responses and autoimmune diseases.
Despite its predicted molecular weight, PD-1 often migrates at a higher molecular weight in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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